GlycoSens™ Bicarbonate Assay Kit

The GlycoSens™ Bicarbonate Assay Kit utilizes a highly stable detection system tailored for accurate profiling of bicarbonate. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Basal Metabolic Series

Bicarbonate

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Target bicarbonate in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target bicarbonate.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Bicarbonate

Bicarbonate

Bicarbonate is an integral element required for robust cellular function and metabolism. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. Moreover, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Specifically engineered for evaluating bicarbonate dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.