GlycoSens™ Mitochondrial Respiratory Chain Complex II Assay Kit, Spectrophotometry

The GlycoSens™ Mitochondrial Respiratory Chain Complex II Assay Kit delivers a robust spectrophotometric protocol for assessing mitochondrial respiratory chain complex II activity. The optimized reaction system minimizes background interference for streamlined workflow efficiency.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex II

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

50 μL

Succinate-coenzyme Q reductase (Complex II) oxidizes succinate while reducing coenzyme Q. Reduced coenzyme Q subsequently transfers electrons to DCPIP, leading to progressive decolorization. Complex II activity is determined by measuring the reduction rate of DCPIP absorbance at 605 nm.

Within 1 hour

605 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex II

Complex II; Mitochondrial Respiratory Chain Complex II Standard; Mitochondrial Respiratory Chain Complex II Reagent; Succinate-CoQ reductase

Mitochondrial respiratory chain complex II acts as an essential biochemical entity within diverse cellular pathways. Its structural integrity and enzymatic turnover are critical determinants of mitochondrial health and reactive oxygen species generation. Evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Tailored for accurate quantification of mitochondrial respiratory chain complex II across enzymology, membrane transport mechanics, and oxidative phosphorylation studies.

Stable Colorimetric Reaction; High Specificity; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.