GlycoSens™ Mitochondrial Respiratory Chain Complex II Assay Kit, Micro-method

The GlycoSens™ Mitochondrial Respiratory Chain Complex II Assay Kit enables precise, direct detection of mitochondrial respiratory chain complex II via micro-volume spectrophotometric measurement. The kit contains ready-to-use reagents optimized for exceptional reproducibility and fast assay turnaround.

Oxidative Phosphorylation Series

Mitochondrial Respiratory Chain Complex II

Mitochondrial Isolation Buffer; Lysis Reagent; Protease Inhibitor Cocktail; Wash Buffer

Isolated mitochondria, cell lysates, tissue homogenates

50 μL

Succinate-Coenzyme Q Reductase (Complex II) oxidizes succinate while reducing coenzyme Q. Reduced coenzyme Q subsequently transfers electrons to DCPIP, leading to progressive decolorization. Complex II activity is determined by measuring the reduction rate of DCPIP absorbance at 605 nm.

Within 1 hour

605 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Mitochondrial Respiratory Chain Complex II

Complex II; Mitochondrial Respiratory Chain Complex II Standard; Mitochondrial Respiratory Chain Complex II Reagent; Succinate-Coenzyme Q Reductase

Found widely across biological samples, mitochondrial respiratory chain complex II is crucial for maintaining systemic homeostasis. It functions as an essential multi-subunit structure within the mitochondrial electron transport chain. Consequently, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Tailored for accurate quantification of mitochondrial respiratory chain complex II across enzymology, succinate dehydrogenase activity, and oxidative phosphorylation studies.

Stable Signal Output; Excellent Reproducibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.