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Quantitative Human 5HTR1B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX344

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Specifications
Product Description
The quantitative human 5HTR1B (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 1B (5HTR1B) levels. 5HTR1B is a G protein-coupled receptor, which binds to serotonin. 5HTR1B plays a role in regulating vasoconstriction. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.062 ng/mL, which can accurately detect low concentrations of 5HTR1B in the sample.
Target
5HTR1B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.062 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor 1B
Alternate Names
5HTR1B; 5-Hydroxytryptamine receptor 1B
Uniprot No.
N-Glycosylation Site
24, 32
Application Notes
Application
The quantitative human 5HTR1B (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated 5HTR1B levels. It is useful for studying the role of 5HTR1B in serotonin signaling.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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