Quantitative Human ABCA1 (Glycosylated) Sandwich ELISA Kit

The quantitative human ABCA1 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter A1 (ABCA1) levels. ABCA1 is a protein that plays a crucial role in lipid transport, specifically by mediating the efflux of cholesterol and phospholipids from cells to apolipoprotein A-I, which is essential for the formation of high-density lipoprotein (HDL). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of ABCA1 in the sample.

ABCA1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.073 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

ATP binding cassette transporter A1

ABCA1; ATP binding cassette transporter A1; CERP; ABC1; Cholesterol efflux regulatory protein

14, 98, 151, 161, 196, 244, 292, 337, 349, 400, 478, 489, 521, 820, 1144, 1294, 1453, 1504, 1637, 2044, 2238

The quantitative human ABCA1 (glycosylated) sandwich ELISA kit utilizes a sandwich ELISA technique, allowing researchers to quantify ABCA1 protein concentrations, which is valuable for studies related to lipid metabolism, cardiovascular disease, and related research areas.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.