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Quantitative Human ABCA1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX117)

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1×48 T
1×96 T

Product Description The quantitative human ABCA1 (glycosylated) sandwich ELISA kit is designed to detect human ATP binding cassette transporter A1 (ABCA1) levels. ABCA1 is a protein that plays a crucial role in lipid transport, specifically by mediating the efflux of cholesterol and phospholipids from cells to apolipoprotein A-I, which is essential for the formation of high-density lipoprotein (HDL). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of ABCA1 in the sample.
Target ABCA1
N-Glycosylation Site 14, 98, 151, 161, 196, 244, 292, 337, 349, 400, 478, 489, 521, 820, 1144, 1294, 1453, 1504, 1637, 2044, 2238
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.073 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name ATP binding cassette transporter A1
Alternate Names ABCA1; ATP binding cassette transporter A1; CERP; ABC1; Cholesterol efflux regulatory protein
Uniprot No. O95477
Application The quantitative human ABCA1 (glycosylated) sandwich ELISA kit utilizes a sandwich ELISA technique, allowing researchers to quantify ABCA1 protein concentrations, which is valuable for studies related to lipid metabolism, cardiovascular disease, and related research areas.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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