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Quantitative Human ADRβ3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX243

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Specifications
Product Description
The quantitative human ADRβ3 (glycosylated) sandwich ELISA kit is designed to detect human adrenergic receptor β3 (ADRβ3) levels. ADRβ3 is a G protein-coupled receptor primarily found in adipose tissue, and it plays a key role in regulating lipolysis and thermogenesis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of ADRβ3 in the sample.
Target
ADRβ3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.128 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adrenergic receptor β3
Alternate Names
ADRβ3; Adrenergic receptor β3; β3AR; B3AR; Beta-3 Adrenoreceptor
Uniprot No.
N-Glycosylation Site
8, 26
Application Notes
Application
The quantitative human ADRβ3 (glycosylated) sandwich ELISA kit is designed to measure the levels of glycosylated ADRβ3 in samples, facilitating research into metabolic processes, obesity, and diabetes. This kit allows researchers to quantify the presence of this specific receptor isoform, providing valuable data for studies related to energy expenditure and metabolic disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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