Quantitative Human ALPPL2 (Glycosylated) Sandwich ELISA Kit

The quantitative human ALPPL2 (glycosylated) sandwich ELISA kit is designed to detect human alkaline phosphatase, placental like protein 2 (ALPPL2) levels. ALPPL2 is an isoenzyme of alkaline phosphatase primarily expressed in germ cells and certain tumors, playing a role in cell proliferation and differentiation. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.123 ng/mL, which can accurately detect low concentrations of ALPPL2 in the sample.

ALPPL2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.123 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Alkaline phosphatase, placental like protein 2

ALPPL2; Alkaline phosphatase, placental like protein 2; ALPG; Alkaline phosphatase Nagao isozyme

141, 268

The quantitative human ALPPL2 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for monitoring germ cell tumors, particularly seminomas and ovarian cancers, and for studying germ cell development. Researchers and clinicians utilize this ELISA to quantify ALPPL2 levels, providing insights into tumor burden and potential diagnostic or prognostic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.