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Quantitative Human ALPPL2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX220)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ALPPL2 (glycosylated) sandwich ELISA kit is designed to detect human alkaline phosphatase, placental like protein 2 (ALPPL2) levels. ALPPL2 is an isoenzyme of alkaline phosphatase primarily expressed in germ cells and certain tumors, playing a role in cell proliferation and differentiation. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.123 ng/mL, which can accurately detect low concentrations of ALPPL2 in the sample.
Target ALPPL2
N-Glycosylation Site 141, 268
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.123 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Alkaline phosphatase, placental like protein 2
Alternate Names ALPPL2; Alkaline phosphatase, placental like protein 2; ALPG; Alkaline phosphatase Nagao isozyme
Uniprot No. P10696
Application The quantitative human ALPPL2 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for monitoring germ cell tumors, particularly seminomas and ovarian cancers, and for studying germ cell development. Researchers and clinicians utilize this ELISA to quantify ALPPL2 levels, providing insights into tumor burden and potential diagnostic or prognostic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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