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Quantitative Human ANO6 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX626

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Specifications
Product Description
The quantitative human ANO6 (glycosylated) sandwich ELISA kit is designed to detect human anoctamin 6 (ANO6) levels. ANO6 is a transmembrane protein that functions as a calcium-activated chloride channel (CaCC) and a phospholipid scramblase. It is involved in regulating chloride ion transport across cell membranes. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.130 ng/mL, which can accurately detect low concentrations of ANO6 in the sample.
Target
ANO6
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.130 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Anoctamin 6
Alternate Names
ANO6; Anoctamin 6; TMEM16F; Transmembrane protein 16F
Uniprot No.
N-Glycosylation Site
329, 361, 493, 777, 790, 802
Application Notes
Application
The quantitative human ANO6 (glycosylated) sandwich ELISA kit provides a quantitative measurement of glycosylated ANO6 levels in biological samples. It is applicable in studies related to ion transport, phospholipid scrambling, and epithelial cell functions. The kit is valuable in fields such as cell biology and physiology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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