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Quantitative Human CHRM3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX284)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CHRM3 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, muscarinic 3 (CHRM3) levels. CHRM3 is a protein that functions as a G protein-coupled receptor. This receptor is activated by acetylcholine, which plays a role in smooth muscle contraction, secretion, and neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.592 ng/mL, which can accurately detect low concentrations of CHRM3 in the sample.
Target CHRM3
N-Glycosylation Site 5, 6, 15, 41, 48
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.592 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.5 ng/mL-45 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cholinergic receptor, muscarinic 3
Alternate Names CHRM3; Cholinergic receptor, muscarinic 3; CHR-M3; HM3; M-AChRM3; Muscarinic acetylcholine receptor M3
Uniprot No. P20309
Application The quantitative human CHRM3 (glycosylated) sandwich ELISA kit is used to determine CHRM3 levels in biological samples. It can be used in studies examining asthma, chronic obstructive pulmonary disease, and bladder dysfunctions. The kit is valuable in pharmacological research.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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