Quantitative Human CHRM3 (Glycosylated) Sandwich ELISA Kit

The quantitative human CHRM3 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, muscarinic 3 (CHRM3) levels. CHRM3 is a protein that functions as a G protein-coupled receptor. This receptor is activated by acetylcholine, which plays a role in smooth muscle contraction, secretion, and neurotransmission. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.592 ng/mL, which can accurately detect low concentrations of CHRM3 in the sample.

CHRM3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.592 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-45 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cholinergic receptor, muscarinic 3

CHRM3; Cholinergic receptor, muscarinic 3; CHR-M3; HM3; M-AChRM3; Muscarinic acetylcholine receptor M3

5, 6, 15, 41, 48

The quantitative human CHRM3 (glycosylated) sandwich ELISA kit is used to determine CHRM3 levels in biological samples. It can be used in studies examining asthma, chronic obstructive pulmonary disease, and bladder dysfunctions. The kit is valuable in pharmacological research.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.