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Quantitative Human CHST9 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX661

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Specifications
Product Description
The quantitative human CHST9 (glycosylated) sandwich ELISA kit is designed to detect human carbohydrate sulfotransferase 9 (CHST9) levels. CHST9 is an enzyme that catalyzes the transfer of sulfate groups to specific carbohydrate molecules. These sulfated carbohydrates are important components of glycosaminoglycans, which are complex sugar molecules found on cell surfaces and in the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.258 ng/mL, which can accurately detect low concentrations of CHST9 in the sample.
Target
CHST9
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.258 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.25 ng/mL-40 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Carbohydrate sulfotransferase 9
Alternate Names
CHST9; Carbohydrate sulfotransferase 9
Uniprot No.
N-Glycosylation Site
159, 243, 324, 437
Application Notes
Application
The quantitative human CHST9 (glycosylated) sandwich ELISA kit is designed to determine the CHST9 levels in biological samples. The kit is applicable in studies of carbohydrate metabolism, glycosaminoglycan synthesis, and cell signaling. It is useful in fields such as biochemistry and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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