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Quantitative Human COL1α2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX195)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL1α2 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 1 α2 (COL1α2) levels. COL1α2 is a subunit of type I collagen, the most abundant collagen in the body, which provides structural support to various tissues, including skin, bone, and tendons. It is crucial for tissue integrity and wound healing. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.252 ng/mL, which can accurately detect low concentrations of COL1α2 in the sample.
Target COL1α2
N-Glycosylation Site 1267
O-Glycosylation Site 177
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.252 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-45 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 1 α2
Alternate Names COL1α2; Collagen type 1 α2; COL1-A2; OI4; Collagen α2(I) chain; Osteogenesis imperfecta type IV
Uniprot No. P08123
Application The quantitative human COL1α2 (glycosylated) sandwich ELISA kit is valuable in research investigating fibrosis, wound healing, bone metabolism, and connective tissue disorders. Researchers utilize this ELISA to quantify COL1α2 levels, providing insights into its role in tissue remodeling and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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