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Quantitative Human COL2α1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX150)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL2α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 2 α1 (COL2α1) levels. COL2α1 is the primary collagen found in cartilage, playing a crucial role in providing tensile strength and structural integrity to this tissue. It is essential for joint function and skeletal development. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.171 ng/mL, which can accurately detect low concentrations of COL2α1 in the sample.
Target COL2α1
N-Glycosylation Site 1388
O-Glycosylation Site 190, 287, 299, 308, 374, 608, 620, 1130
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.171 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 2 α1
Alternate Names COL2α1; Collagen type 2 α1; COL2-A1; SEDC; Chondrocalcin; Collagen α1(II) chain
Uniprot No. P02458
Application The quantitative human COL2α1 (glycosylated) sandwich ELISA kit is valuable in research investigating osteoarthritis, skeletal dysplasia, and other cartilage-related disorders. Researchers utilize this ELISA to quantify COL2α1 levels, providing insights into its role in cartilage homeostasis and disease progression.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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