Quantitative Human COL2α1 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL2α1 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 2 α1 (COL2α1) levels. COL2α1 is the primary collagen found in cartilage, playing a crucial role in providing tensile strength and structural integrity to this tissue. It is essential for joint function and skeletal development. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.171 ng/mL, which can accurately detect low concentrations of COL2α1 in the sample.

COL2α1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Serum, plasma

100 μL

0.171 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 2 α1

COL2α1; Collagen type 2 α1; COL2-A1; SEDC; Chondrocalcin; Collagen α1(II) chain

1388

190, 287, 299, 308, 374, 608, 620, 1130

The quantitative human COL2α1 (glycosylated) sandwich ELISA kit is valuable in research investigating osteoarthritis, skeletal dysplasia, and other cartilage-related disorders. Researchers utilize this ELISA to quantify COL2α1 levels, providing insights into its role in cartilage homeostasis and disease progression.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.