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Quantitative Human COL6α3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX234)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL6α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 6 α3 (COL6α3) levels. COL6α3 is a subunit of type VI collagen, a microfibrillar collagen that forms a network in the extracellular matrix, playing a role in cell adhesion and tissue integrity. Its alterations are implicated in certain muscular dystrophies and connective tissue disorders. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 12.528 pg/mL, which can accurately detect low concentrations of COL6α3 in the sample.
Target COL6α3
N-Glycosylation Site 108, 116, 202, 251, 2079, 2331, 2558, 2677, 2861, 3037
O-Glycosylation Site 2103, 2209, 2212, 2322, 2337
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 12.528 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 pg/mL-2000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 6 α3
Alternate Names COL6α3; Collagen type 6 α3; COL6-A3; Collagen α3(VI) chain
Uniprot No. P12111
Application The quantitative human COL6α3 (glycosylated) sandwich ELISA kit is valuable in research investigating muscular dystrophies, particularly those related to COL6 mutations, and in studies of extracellular matrix biology and fibrosis. Researchers utilize this ELISA to quantify COL6α3 levels, providing insights into its role in tissue structure and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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