Quantitative Human COL6α3 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL6α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 6 α3 (COL6α3) levels. COL6α3 is a subunit of type VI collagen, a microfibrillar collagen that forms a network in the extracellular matrix, playing a role in cell adhesion and tissue integrity. Its alterations are implicated in certain muscular dystrophies and connective tissue disorders. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 12.528 pg/mL, which can accurately detect low concentrations of COL6α3 in the sample.

COL6α3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

12.528 pg/mL

Quantitative sandwich ELISA

50 pg/mL-2000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 6 α3

COL6α3; Collagen type 6 α3; COL6-A3; Collagen α3(VI) chain

108, 116, 202, 251, 2079, 2331, 2558, 2677, 2861, 3037

2103, 2209, 2212, 2322, 2337

The quantitative human COL6α3 (glycosylated) sandwich ELISA kit is valuable in research investigating muscular dystrophies, particularly those related to COL6 mutations, and in studies of extracellular matrix biology and fibrosis. Researchers utilize this ELISA to quantify COL6α3 levels, providing insights into its role in tissue structure and potential diagnostic or therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.