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Quantitative Human CRHR1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX380)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CRHR1 (glycosylated) sandwich ELISA kit is designed to detect human corticotropin releasing hormone receptor 1 (CRHR1) levels. CRHR1 is a G protein-coupled receptor, which binds to corticotropin-releasing hormone (CRH). CRHR1 plays a role in regulating the stress response. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of CRHR1 in the sample.
Target CRHR1
N-Glycosylation Site 38, 45, 78, 90, 98
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.094 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Corticotropin releasing hormone receptor 1
Alternate Names CRHR1; Corticotropin releasing hormone receptor 1; CRF1; CRFR1; Corticotropin-releasing factor receptor 1
Uniprot No. P34998
Application The quantitative human CRHR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CRHR1 levels. It is useful for studying the role of CRHR1 in the hypothalamic-pituitary-adrenal (HPA) axis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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