Quantitative Human CRHR1 (Glycosylated) Sandwich ELISA Kit

The quantitative human CRHR1 (glycosylated) sandwich ELISA kit is designed to detect human corticotropin releasing hormone receptor 1 (CRHR1) levels. CRHR1 is a G protein-coupled receptor, which binds to corticotropin-releasing hormone (CRH). CRHR1 plays a role in regulating the stress response. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of CRHR1 in the sample.

CRHR1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.094 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Corticotropin releasing hormone receptor 1

CRHR1; Corticotropin releasing hormone receptor 1; CRF1; CRFR1; Corticotropin-releasing factor receptor 1

38, 45, 78, 90, 98

The quantitative human CRHR1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CRHR1 levels. It is useful for studying the role of CRHR1 in the hypothalamic-pituitary-adrenal (HPA) axis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.