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Quantitative Human CRTAP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX95)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CRTAP (glycosylated) sandwich ELISA kit is designed to detect human cartilage associated protein (CRTAP) levels. CRTAP is an endoplasmic reticulum-resident protein that plays a crucial role in collagen prolyl 3-hydroxylation and is essential for normal bone and cartilage development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.103 ng/mL, which can accurately detect low concentrations of CRTAP in the sample.
Target CRTAP
N-Glycosylation Site 87, 363
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.103 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cartilage associated protein
Alternate Names CRTAP; Cartilage associated protein; CASP; Leprecan-like 3
Uniprot No. O75718
Application The quantitative human CRTAP (glycosylated) sandwich ELISA kit is valuable for research investigating osteogenesis imperfecta and other bone and cartilage disorders. The quantitative data obtained from this kit allows for precise analysis of CRTAP levels, contributing to a better understanding of its role in collagen biosynthesis and skeletal development.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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