Quantitative Human CRTAP (Glycosylated) Sandwich ELISA Kit

The quantitative human CRTAP (glycosylated) sandwich ELISA kit is designed to detect human cartilage associated protein (CRTAP) levels. CRTAP is an endoplasmic reticulum-resident protein that plays a crucial role in collagen prolyl 3-hydroxylation and is essential for normal bone and cartilage development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.103 ng/mL, which can accurately detect low concentrations of CRTAP in the sample.

CRTAP

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.103 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cartilage associated protein

CRTAP; Cartilage associated protein; CASP; Leprecan-like 3

87, 363

The quantitative human CRTAP (glycosylated) sandwich ELISA kit is valuable for research investigating osteogenesis imperfecta and other bone and cartilage disorders. The quantitative data obtained from this kit allows for precise analysis of CRTAP levels, contributing to a better understanding of its role in collagen biosynthesis and skeletal development.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.