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Quantitative Human ECE1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX416

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Specifications
Product Description
The quantitative human ECE1 (glycosylated) sandwich ELISA kit is designed to detect human endothelin converting enzyme 1 (ECE1) levels. ECE1 is a metalloprotease that catalyzes the final step in the production of endothelin-1. Endothelin-1 is a potent vasoconstrictor peptide. ECE1 plays a crucial role in regulating blood pressure and vascular tone. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 12.253 pg/mL, which can accurately detect low concentrations of ECE1 in the sample.
Target
ECE1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
12.253 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Endothelin converting enzyme 1
Alternate Names
ECE1; Endothelin converting enzyme 1
Uniprot No.
N-Glycosylation Site
166, 187, 210, 270, 316, 362, 383, 539, 632, 651
Application Notes
Application
The quantitative human ECE1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated ECE1 levels in samples. This measurement is valuable in research related to cardiovascular physiology and disease. The kit is applied in studies of conditions such as hypertension and heart failure.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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