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Quantitative Human ECE1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX416)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ECE1 (glycosylated) sandwich ELISA kit is designed to detect human endothelin converting enzyme 1 (ECE1) levels. ECE1 is a metalloprotease that catalyzes the final step in the production of endothelin-1. Endothelin-1 is a potent vasoconstrictor peptide. ECE1 plays a crucial role in regulating blood pressure and vascular tone. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 12.253 pg/mL, which can accurately detect low concentrations of ECE1 in the sample.
Target ECE1
N-Glycosylation Site 166, 187, 210, 270, 316, 362, 383, 539, 632, 651
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 12.253 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 pg/mL-1500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Endothelin converting enzyme 1
Alternate Names ECE1; Endothelin converting enzyme 1
Uniprot No. P42892
Application The quantitative human ECE1 (glycosylated) sandwich ELISA kit is designed to measure glycosylated ECE1 levels in samples. This measurement is valuable in research related to cardiovascular physiology and disease. The kit is applied in studies of conditions such as hypertension and heart failure.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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