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Quantitative Human EFNA4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX460

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Specifications
Product Description
The quantitative human EFNA4 (glycosylated) sandwich ELISA kit is designed to detect human ephrin A4 (EFNA4) levels. EFNA4 is another member of the ephrin protein family, which interacts with Eph receptors. EFNA4 signaling is involved in cell-cell communication. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.138 ng/mL, which can accurately detect low concentrations of EFNA4 in the sample.
Target
EFNA4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.138 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin A4
Alternate Names
EFNA4; Ephrin A4; EPLG4; LERK4; EPH-related receptor tyrosine kinase ligand 4
Uniprot No.
N-Glycosylation Site
33
Application Notes
Application
The quantitative human EFNA4 (glycosylated) sandwich ELISA kit is designed to measure glycosylated EFNA4 levels in samples. This measurement is valuable in research related to cell signaling. The kit is applied in studies of developmental processes, which helps in understanding the role of EFNA4.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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