Quantitative Human ENPP1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX307)

Product Description	The quantitative human ENPP1 (glycosylated) sandwich ELISA kit is designed to detect human ectonucleotide pyrophosphatase 1 (ENPP1) levels. ENPP1 is a transmembrane glycoprotein, which has multiple enzymatic activities. ENPP1 can hydrolyze various nucleotides and phosphodiester bonds. It plays a role in regulating nucleotide levels. It is also involved in insulin signaling and bone mineralization. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.082 ng/mL, which can accurately detect low concentrations of ENPP1 in the sample.
Target	ENPP1
N-Glycosylation Site	179, 285, 341, 477, 585, 643, 700, 731, 748
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.082 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Ectonucleotide pyrophosphatase 1
Alternate Names	ENPP1; Ectonucleotide pyrophosphatase 1; M6S1; NPP1; PDNP1; Membrane component chromosome 6 surface marker 1
Uniprot No.	P22413

Application	The quantitative human ENPP1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ENPP1 levels in biological samples. It is useful for studying the role of ENPP1 in metabolic regulation.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 95-110%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.