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Quantitative Human FUT2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX546

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Specifications
Product Description
The quantitative human FUT2 (glycosylated) sandwich ELISA kit is designed to detect human fucosyltransferase 2 (FUT2) levels. FUT2 is an enzyme that catalyzes the addition of fucose sugars to certain carbohydrate structures. This modification affects the expression of specific blood group antigens, including the H antigen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.143 ng/mL, which can accurately detect low concentrations of FUT2 in the sample.
Target
FUT2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.143 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Fucosyltransferase 2
Alternate Names
FUT2; Fucosyltransferase 2; SE; Secretor blood group alpha-2-fucosyltransferase
Uniprot No.
N-Glycosylation Site
188, 282, 308
Application Notes
Application
The quantitative human FUT2 (glycosylated) sandwich ELISA kit is a valuable tool for research in glycobiology, genetics, and infectious diseases, particularly in studies of blood group antigens and host-microbe interactions. Researchers use it to measure FUT2 levels in tissues. The kit has applications in studies of susceptibility to infections.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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