Quantitative Human HSD11b1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX348)

Name: Quantitative Human HSD11b1 (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX348
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human HSD11b1 (glycosylated) sandwich ELISA kit is designed to detect human 11-β-hydroxysteroid dehydrogenase type 1 (HSD11b1) levels. HSD11B1 is an enzyme, which catalyzes the conversion of cortisone to cortisol. HSD11B1 plays a role in regulating glucocorticoid action. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.676 ng/mL, which can accurately detect low concentrations of HSD11b1 in the sample.
Target	HSD11b1
N-Glycosylation Site	123, 162, 207
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.676 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	2.5 ng/mL-85 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	11-β-Hydroxysteroid dehydrogenase type 1
Alternate Names	HSD11b1; 11-β-Hydroxysteroid dehydrogenase type 1; 11-β-HSD1; HSD11; SDR26C1; Corticosteroid 11-β-dehydrogenase isozyme 1
Uniprot No.	P28845

Application	The quantitative human HSD11b1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HSD11B1 levels. It is useful for studying the role of HSD11B1 in cortisol production.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 80-90%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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