Quantitative Human HSD11b1 (Glycosylated) Sandwich ELISA Kit

The quantitative human HSD11b1 (glycosylated) sandwich ELISA kit is designed to detect human 11-β-hydroxysteroid dehydrogenase type 1 (HSD11b1) levels. HSD11B1 is an enzyme, which catalyzes the conversion of cortisone to cortisol. HSD11B1 plays a role in regulating glucocorticoid action. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.676 ng/mL, which can accurately detect low concentrations of HSD11b1 in the sample.

HSD11b1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.676 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-85 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

11-β-Hydroxysteroid dehydrogenase type 1

HSD11b1; 11-β-Hydroxysteroid dehydrogenase type 1; 11-β-HSD1; HSD11; SDR26C1; Corticosteroid 11-β-dehydrogenase isozyme 1

123, 162, 207

The quantitative human HSD11b1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HSD11B1 levels. It is useful for studying the role of HSD11B1 in cortisol production.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-90%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.