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Quantitative Human MGAT5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX542)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MGAT5 (glycosylated) sandwich ELISA kit is designed to detect human mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase (MGAT5) levels. MGAT5 is an enzyme involved in the glycosylation process, which catalyzes the formation of β-1,6-branched N-glycans. These glycan structures can influence protein folding, stability, and functions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of MGAT5 in the sample.
Target MGAT5
N-Glycosylation Site 110, 115, 118, 334, 433, 447
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.097 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase
Alternate Names MGAT5; Mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase; GNT-V; GGNT5; Mannoside acetylglucosaminyltransferase 5
Uniprot No. Q09328
Application The quantitative human MGAT5 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and glycobiology, particularly in studies of protein glycosylation. Researchers use it to measure MGAT5 levels in cells and tissues. The kit has applications in studies of cancer, cell signaling, and protein folding.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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