Quantitative Human MGAT5 (Glycosylated) Sandwich ELISA Kit

The quantitative human MGAT5 (glycosylated) sandwich ELISA kit is designed to detect human mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase (MGAT5) levels. MGAT5 is an enzyme involved in the glycosylation process, which catalyzes the formation of β-1,6-branched N-glycans. These glycan structures can influence protein folding, stability, and functions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of MGAT5 in the sample.

MGAT5

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.097 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase

MGAT5; Mannosyl α-1,6-glycoprotein β-1,6-N-acetylglucosaminyltransferase; GNT-V; GGNT5; Mannoside acetylglucosaminyltransferase 5

110, 115, 118, 334, 433, 447

The quantitative human MGAT5 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and glycobiology, particularly in studies of protein glycosylation. Researchers use it to measure MGAT5 levels in cells and tissues. The kit has applications in studies of cancer, cell signaling, and protein folding.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.