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Quantitative Human MUC6 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX651

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Specifications
Product Description
The quantitative human MUC6 (glycosylated) sandwich ELISA kit is designed to detect human mucin 6 (MUC6) levels. MUC6 is a glycoprotein that is secreted by mucous cells in various tissues, particularly in the gastrointestinal tract. MUC6 is a major component of mucus, forming a protective barrier on epithelial surfaces. It plays a crucial role in maintaining the integrity of the mucosal layer and protecting against pathogens, mechanical stress, and chemical damage. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.124 ng/mL, which can accurately detect low concentrations of MUC6 in the sample.
Target
MUC6
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.124 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Mucin 6
Alternate Names
MUC6; Mucin 6
Uniprot No.
N-Glycosylation Site
268, 486, 659, 975, 1179
Application Notes
Application
The quantitative human MUC6 (glycosylated) sandwich ELISA kit provides a quantitative measurement of glycosylated MUC6 levels in biological samples. It is applicable in studies of mucosal biology, epithelial protection, and gastrointestinal functions. The kit is valuable in fields such as cell biology and gastroenterology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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