Quantitative Human NCCT (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX471)

Name: Quantitative Human NCCT (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX471
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human NCCT (glycosylated) sandwich ELISA kit is designed to detect human Na-Cl cotransporter (NCCT) levels. NCCT is a membrane protein located in the distal convoluted tubule of the kidney, which plays a crucial role in regulating sodium chloride reabsorption. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of NCCT in the sample.
Target	NCCT
N-Glycosylation Site	406, 426
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.125 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Na-Cl cotransporter
Alternate Names	NCCT; Na-Cl cotransporter; SLC12A3; TSC; NCC
Uniprot No.	P55017

Application	The quantitative human NCCT (glycosylated) sandwich ELISA kit is used to measure glycosylated NCCT levels in samples. This measurement is important in research related to kidney functions and electrolyte balance. The kit is applied in studies of conditions affecting blood pressure, which helps in understanding the role of NCCT.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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