Quantitative Human NCCT (Glycosylated) Sandwich ELISA Kit

The quantitative human NCCT (glycosylated) sandwich ELISA kit is designed to detect human Na-Cl cotransporter (NCCT) levels. NCCT is a membrane protein located in the distal convoluted tubule of the kidney, which plays a crucial role in regulating sodium chloride reabsorption. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of NCCT in the sample.

NCCT

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Na-Cl cotransporter

NCCT; Na-Cl cotransporter; SLC12A3; TSC; NCC

406, 426

The quantitative human NCCT (glycosylated) sandwich ELISA kit is used to measure glycosylated NCCT levels in samples. This measurement is important in research related to kidney functions and electrolyte balance. The kit is applied in studies of conditions affecting blood pressure, which helps in understanding the role of NCCT.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.