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Quantitative Human PAR4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX752

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Specifications
Product Description
The quantitative human PAR4 (glycosylated) sandwich ELISA kit is designed to detect human protease activated receptor 4 (PAR4) levels. PAR4 is a G protein-coupled receptor that is activated by proteases. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.135 ng/mL, which can accurately detect low concentrations of PAR4 in the sample.
Target
PAR4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.135 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protease activated receptor 4
Alternate Names
PAR4; Protease activated receptor 4; F2RL3; PAR-4; Coagulation factor II receptor-like 3
Uniprot No.
N-Glycosylation Site
56
Application Notes
Application
The quantitative human PAR4 (glycosylated) sandwich ELISA kit is developed for the quantitative analysis of glycosylated PAR4 in samples. This measurement is valuable for research on platelet functions and blood clotting. The kit also has applications in investigations of thrombosis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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