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Quantitative Human PCDH15 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX749

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Specifications
Product Description
The quantitative human PCDH15 (glycosylated) sandwich ELISA kit is designed to detect human protocadherin 15 (PCDH15) levels. PCDH15 is a cell adhesion molecule that is a member of the cadherin superfamily. PCDH15 plays a role in the development of the inner ear. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.157 ng/mL, which can accurately detect low concentrations of PCDH15 in the sample.
Target
PCDH15
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.157 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Protocadherin 15
Alternate Names
PCDH15; Protocadherin 15; CDHR15; Cadherin-related family member 15
Uniprot No.
N-Glycosylation Site
52, 97, 201, 419, 559, 662, 724, 768, 821, 851, 1064, 1084, 1175
Application Notes
Application
The quantitative human PCDH15 (glycosylated) sandwich ELISA kit is useful for measuring PCDH15 levels in the samples. This measurement is relevant to research on hearing and inner ear development. The kit also has potential applications in investigations of hearing loss.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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