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Quantitative Human RAMP2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX79

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Specifications
Product Description
The quantitative human RAMP2 (glycosylated) sandwich ELISA kit is designed to detect human receptor activity modifying protein 2 (RAMP2) levels. RAMP2 is a membrane-associated protein that modulates the function of G protein-coupled receptors, particularly calcitonin gene-related peptide (CGRP) receptors, affecting vascular function and pain signaling. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of RAMP2 in the sample.
Target
RAMP2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.099 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Receptor activity modifying protein 2
Alternate Names
RAMP2; Receptor activity modifying protein 2; Calcitonin-receptor-like receptor activity-modifying protein 2
Uniprot No.
N-Glycosylation Site
130
Application Notes
Application
The quantitative human RAMP2 (glycosylated) sandwich ELISA kit is valuable for research investigating vascular diseases, migraine, and other conditions related to CGRP signaling. The quantitative data obtained from this kit allows for precise analysis of RAMP2 levels, contributing to a better understanding of its role in these physiological and pathological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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