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Quantitative Human SEMA4A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX970

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human SEMA4A (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 4A (SEMA4A) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of SEMA4A in the sample.
Target
SEMA4A
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.085 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.32 ng/mL-20 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Semaphorin 4A
Alternate Names
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; CORD10; RP35; SEMAB; Semaphorin 4A precursor; Semaphorin B; SEMA4A
Uniprot No.
Q9H3S1
N-Glycosylation Site
120, 135, 496, 607
Application Notes
Application
The quantitative human SEMA4A (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of SEMA4A, helping to understand its role in cardiovascular.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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