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Quantitative Human SEMA4D (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX971

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human SEMA4D (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 4D (SEMA4D) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 31.322 pg/mL, which can accurately detect low concentrations of SEMA4D in the sample.
Target
SEMA4D
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
31.322 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
130 pg/mL-8000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Semaphorin 4D
Alternate Names
Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; CD100; CD100 antigen; COLL4; Collapsin 4; Leukocyte activation antigen CD100; Semaphorin 4D; Semaphorin C like 2; SEMA4D
Uniprot No.
Q92854
N-Glycosylation Site
49, 77, 139, 191, 329, 379, 419, 613, 632
Application Notes
Application
The quantitative human SEMA4D (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of SEMA4D, helping to understand its role in neuroscience.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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