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Quantitative Human SPARC (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1043

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Specifications
Product Description
The quantitative human SPARC (glycosylated) sandwich ELISA kit is designed to detect human secreted protein acidic and rich in cysteine (SPARC) levels. It is suitable for various biological samples such as tissue homogenates, serum, plasma, or urine. Its sensitivity is 15.856 ng/mL, which can accurately detect low concentrations of SPARC in the sample.
Target
SPARC
Sample Types
Tissue homogenates, serum, plasma, urine
Sample Volume
50 μL-100 μL
Sensitivity
15.856 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
65 ng/mL-4000 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Secreted protein acidic and rich in cysteine
Alternate Names
Secreted protein, acidic, cysteine-rich; Basement-membrane protein 40; BM40; Cysteine rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; SPARC
Uniprot No.
N-Glycosylation Site
116
Application Notes
Application
The quantitative human SPARC (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of SPARC, helping to understand its role in cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 95-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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