Quantitative Human SPARC (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX1043)

Product Description	The quantitative human SPARC (glycosylated) sandwich ELISA kit is designed to detect human secreted protein acidic and rich in cysteine (SPARC) levels. It is suitable for various biological samples such as tissue homogenates, serum, plasma, or urine. Its sensitivity is 15.856 ng/mL, which can accurately detect low concentrations of SPARC in the sample.
Target	SPARC
N-Glycosylation Site	116
Sample Types	Tissue homogenates, serum, plasma, urine
Sample Volume	50 μL-100 μL
Sensitivity	15.856 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	65 ng/mL-4000 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Secreted protein acidic and rich in cysteine
Alternate Names	Secreted protein, acidic, cysteine-rich; Basement-membrane protein 40; BM40; Cysteine rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; SPARC
Uniprot No.	P09486

Application	The quantitative human SPARC (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of SPARC, helping to understand its role in cancer.
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-95%; Plasma sample: n=4, 95-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.