Quantitative Human SPARCL1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX590)

Product Description	The quantitative human SPARCL1 (glycosylated) sandwich ELISA kit is designed to detect human sparc like protein 1 (SPARCL1) levels. SPARCL1 is found in the extracellular matrix, which interacts with various matrix components. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.165 ng/mL, which can accurately detect low concentrations of SPARCL1 in the sample.
Target	SPARCL1
N-Glycosylation Site	169, 176, 196, 280, 412, 476
O-Glycosylation Site	31, 40, 44, 116, 331, 398
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.165 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Sparc like protein 1
Alternate Names	SPARCL1; Sparc like protein 1; SC1; Mast9; High endothelial venule protein
Uniprot No.	Q14515

Application	The quantitative human SPARCL1 (glycosylated) sandwich ELISA kit is designed for the quantitative determination of SPARCL1 concentrations in biological specimens. This measurement is crucial for studies investigating cell-matrix interactions. It also contributes to understanding the role of SPARCL1 in tissue remodeling.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 90-105%; Plasma sample: n=4, 90-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.