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Quantitative Human SPARCL1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX590

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Specifications
Product Description
The quantitative human SPARCL1 (glycosylated) sandwich ELISA kit is designed to detect human sparc like protein 1 (SPARCL1) levels. SPARCL1 is found in the extracellular matrix, which interacts with various matrix components. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.165 ng/mL, which can accurately detect low concentrations of SPARCL1 in the sample.
Target
SPARCL1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.165 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sparc like protein 1
Alternate Names
SPARCL1; Sparc like protein 1; SC1; Mast9; High endothelial venule protein
Uniprot No.
N-Glycosylation Site
169, 176, 196, 280, 412, 476
O-Glycosylation Site
31, 40, 44, 116, 331, 398
Application Notes
Application
The quantitative human SPARCL1 (glycosylated) sandwich ELISA kit is designed for the quantitative determination of SPARCL1 concentrations in biological specimens. This measurement is crucial for studies investigating cell-matrix interactions. It also contributes to understanding the role of SPARCL1 in tissue remodeling.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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