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Quantitative Human SULF2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX679)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SULF2 (glycosylated) sandwich ELISA kit is designed to detect human sulfatase 2 (SULF2) levels. SULF2 is an enzyme, which modifies heparan sulfate proteoglycans. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of SULF2 in the sample.
Target SULF2
N-Glycosylation Site 65, 112, 132, 149, 171, 198, 241, 561, 608, 717, 754, 764
O-Glycosylation Site 583
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sulfatase 2
Alternate Names SULF2; Sulfatase 2; Extracellular sulfatase Sulf-2
Uniprot No. Q8IWU5
Application The quantitative human SULF2 (glycosylated) sandwich ELISA kit is used to determine SULF2 concentrations in biological specimens. This measurement is crucial for studies investigating heparan sulfate proteoglycans. It also contributes to understanding the role of SULF2 in cell signaling. Furthermore, it is valuable in research exploring cancer biology and developmental processes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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