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Quantitative Human SULF2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX679

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Specifications
Product Description
The quantitative human SULF2 (glycosylated) sandwich ELISA kit is designed to detect human sulfatase 2 (SULF2) levels. SULF2 is an enzyme, which modifies heparan sulfate proteoglycans. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of SULF2 in the sample.
Target
SULF2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sulfatase 2
Alternate Names
SULF2; Sulfatase 2; Extracellular sulfatase Sulf-2
Uniprot No.
N-Glycosylation Site
65, 112, 132, 149, 171, 198, 241, 561, 608, 717, 754, 764
O-Glycosylation Site
583
Application Notes
Application
The quantitative human SULF2 (glycosylated) sandwich ELISA kit is used to determine SULF2 concentrations in biological specimens. This measurement is crucial for studies investigating heparan sulfate proteoglycans. It also contributes to understanding the role of SULF2 in cell signaling. Furthermore, it is valuable in research exploring cancer biology and developmental processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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