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Quantitative Human TMPRSS2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX41)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human TMPRSS2 (glycosylated) sandwich ELISA kit is designed to detect human transmembrane protease, serine 2 (TMPRSS2) levels. TMPRSS2 is a protein that functions as a serine protease, playing a key role in the activation of viral spike proteins, notably in coronaviruses like SARS-CoV-2, facilitating viral entry into host cells. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of TMPRSS2 in the sample.
Target TMPRSS2
N-Glycosylation Site 213, 249
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Transmembrane protease, serine 2
Alternate Names TMPRSS2; Transmembrane protease, serine 2; PRSS10; Serine protease 10
Uniprot No. O15393
Application The quantitative human TMPRSS2 (glycosylated) sandwich ELISA kit is utilized for the precise measurement of glycosylated TMPRSS2 levels in biological samples, aiding in research related to viral infections and other conditions where TMPRSS2 expression is relevant. This assay allows researchers to quantify TMPRSS2, which can be important for understanding disease progression and the efficacy of potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 95-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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