Quantitative Human TPP1 (Glycosylated) Sandwich ELISA Kit

The quantitative human TPP1 (glycosylated) sandwich ELISA kit is designed to detect human tripeptidyl peptidase 1 (TPP1) levels. TPP1 is a lysosomal enzyme that plays a crucial role in the degradation of peptides, particularly in neuronal cells. It is essential for lysosomal function and the proper turnover of intracellular proteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of TPP1 in the sample.

TPP1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Tripeptidyl peptidase 1

TPP1; Tripeptidyl peptidase 1; CLN2; LPIC; Lysosomal pepstatin-insensitive protease

210, 222, 286, 313, 443

The quantitative human TPP1 (glycosylated) sandwich ELISA kit is valuable for researchers investigating neuronal ceroid lipofuscinosis (NCL), a lysosomal storage disorder caused by TPP1 deficiency. By quantifying TPP1, researchers can gain insights into disease pathogenesis, monitor disease progression, and evaluate potential therapeutic interventions, such as enzyme replacement therapy.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.