Quantitative Human UGT1A1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX306)

Product Description	The quantitative human UGT1A1 (glycosylated) sandwich ELISA kit is designed to detect human UDP glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1) levels. UGT1A1 is an enzyme involved in the metabolism of various compounds. It catalyzes the conjugation of glucuronic acid to substrates. These substrates include bilirubin, drugs, and other xenobiotics. UGT1A1 plays a crucial role in drug detoxification and bilirubin metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.937 ng/mL, which can accurately detect low concentrations of UGT1A1 in the sample.
Target	UGT1A1
N-Glycosylation Site	102, 295, 347
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.937 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	2.5 ng/mL-85 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	UDP glucuronosyltransferase 1 family, polypeptide A1
Alternate Names	UGT1A1; UDP glucuronosyltransferase 1 family, polypeptide A1; UGT1; GNT1; UDPGT; UDP-glucuronosyltransferase 1
Uniprot No.	P22309

Application	The quantitative human UGT1A1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated UGT1A1 in biological samples. It is particularly relevant in studies of drug metabolism.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 90-110%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.