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Quantitative Human UGT1A1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX306

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Specifications
Product Description
The quantitative human UGT1A1 (glycosylated) sandwich ELISA kit is designed to detect human UDP glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1) levels. UGT1A1 is an enzyme involved in the metabolism of various compounds. It catalyzes the conjugation of glucuronic acid to substrates. These substrates include bilirubin, drugs, and other xenobiotics. UGT1A1 plays a crucial role in drug detoxification and bilirubin metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.937 ng/mL, which can accurately detect low concentrations of UGT1A1 in the sample.
Target
UGT1A1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.937 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.5 ng/mL-85 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
UDP glucuronosyltransferase 1 family, polypeptide A1
Alternate Names
UGT1A1; UDP glucuronosyltransferase 1 family, polypeptide A1; UGT1; GNT1; UDPGT; UDP-glucuronosyltransferase 1
Uniprot No.
N-Glycosylation Site
102, 295, 347
Application Notes
Application
The quantitative human UGT1A1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated UGT1A1 in biological samples. It is particularly relevant in studies of drug metabolism.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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