Quantitative Human VIM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX206)

Name: Quantitative Human VIM (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX206
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human VIM (glycosylated) sandwich ELISA kit is designed to detect human vimentin (VIM) levels. VIM is an intermediate filament protein expressed in mesenchymal cells, playing a critical role in maintaining cell shape, integrity, and motility. It is involved in various cellular processes, including wound healing and epithelial-mesenchymal transition (EMT). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.782 ng/mL, which can accurately detect low concentrations of VIM in the sample.
Target	VIM
O-Glycosylation Site	7, 33, 34
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.782 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	2.5 ng/mL-100 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Application	The quantitative human VIM (glycosylated) sandwich ELISA kit is valuable in research investigating cancer metastasis, fibrosis, and wound healing, where VIM expression and modifications are relevant. Researchers utilize this ELISA to quantify VIM levels, providing insights into mesenchymal cell activity and potential diagnostic or therapeutic applications.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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