Quantitative Human VIM (Glycosylated) Sandwich ELISA Kit

The quantitative human VIM (glycosylated) sandwich ELISA kit is designed to detect human vimentin (VIM) levels. VIM is an intermediate filament protein expressed in mesenchymal cells, playing a critical role in maintaining cell shape, integrity, and motility. It is involved in various cellular processes, including wound healing and epithelial-mesenchymal transition (EMT). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.782 ng/mL, which can accurately detect low concentrations of VIM in the sample.

VIM

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.782 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Vimentin

VIM; Vimentin

7, 33, 34

The quantitative human VIM (glycosylated) sandwich ELISA kit is valuable in research investigating cancer metastasis, fibrosis, and wound healing, where VIM expression and modifications are relevant. Researchers utilize this ELISA to quantify VIM levels, providing insights into mesenchymal cell activity and potential diagnostic or therapeutic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.