Quantitative Mouse CH25H (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX939)

Product Description	The quantitative mouse CH25H (glycosylated) sandwich ELISA kit is designed to detect mouse cholesterol 25-hydroxylase (CH25H) levels. CH25H is an enzyme that catalyzes the conversion of cholesterol to 25-hydroxycholesterol (25-HC). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.317 ng/mL, which can accurately detect low concentrations of CH25H in the sample.
Target	CH25H
N-Glycosylation Site	5, 163
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.317 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1.55 ng/mL-45 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Mouse
Full Name	Cholesterol 25-hydroxylase
Alternate Names	CH25H; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase
Uniprot No.	Q9Z0F5

Application	The quantitative mouse CH25H (glycosylated) sandwich ELISA kit is used to measure CH25H levels in biological samples. The assay enables the study of CH25H's role in cholesterol metabolism, immune responses, and viral infections.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 90-110%; Plasma sample: n=4, 90-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.