Quantitative Mouse CH25H (Glycosylated) Sandwich ELISA Kit

The quantitative mouse CH25H (glycosylated) sandwich ELISA kit is designed to detect mouse cholesterol 25-hydroxylase (CH25H) levels. CH25H is an enzyme that catalyzes the conversion of cholesterol to 25-hydroxycholesterol (25-HC). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.317 ng/mL, which can accurately detect low concentrations of CH25H in the sample.

CH25H

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.317 ng/mL

Quantitative sandwich ELISA

1.55 ng/mL-45 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Mouse

Cholesterol 25-hydroxylase

CH25H; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase

5, 163

The quantitative mouse CH25H (glycosylated) sandwich ELISA kit is used to measure CH25H levels in biological samples. The assay enables the study of CH25H's role in cholesterol metabolism, immune responses, and viral infections.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.