Quantitative Human CH25H (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX127)

Product Description	The quantitative human CH25H (glycosylated) sandwich ELISA kit is designed to detect human cholesterol-25-hydroxylase (CH25H) levels. CH25H is an enzyme that catalyzes the conversion of cholesterol to 25-hydroxycholesterol (25-HC), playing a crucial role in lipid metabolism and immune regulation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 52.836 pg/mL, which can accurately detect low concentrations of CH25H in the sample.
Target	CH25H
N-Glycosylation Site	5, 163, 189
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	100 μL
Sensitivity	52.836 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	150 pg/mL-6000 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Cholesterol-25-hydroxylase
Alternate Names	CH25H; Cholesterol-25-hydroxylase; Cholesterol 25-monooxygenase
Uniprot No.	O95992

Application	The quantitative human CH25H (glycosylated) sandwich ELISA kit is instrumental in research aimed at understanding the role of CH25H in various physiological and pathological processes, particularly in studies concerning cholesterol metabolism, immune responses, and inflammatory conditions. Researchers utilize this ELISA kit to quantify CH25H, thereby gaining valuable insights into its involvement in health and disease.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.