Quantitative Human CH25H (Glycosylated) Sandwich ELISA Kit

The quantitative human CH25H (glycosylated) sandwich ELISA kit is designed to detect human cholesterol-25-hydroxylase (CH25H) levels. CH25H is an enzyme that catalyzes the conversion of cholesterol to 25-hydroxycholesterol (25-HC), playing a crucial role in lipid metabolism and immune regulation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 52.836 pg/mL, which can accurately detect low concentrations of CH25H in the sample.

CH25H

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

52.836 pg/mL

Quantitative sandwich ELISA

150 pg/mL-6000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cholesterol-25-hydroxylase

CH25H; Cholesterol-25-hydroxylase; Cholesterol 25-monooxygenase

5, 163, 189

The quantitative human CH25H (glycosylated) sandwich ELISA kit is instrumental in research aimed at understanding the role of CH25H in various physiological and pathological processes, particularly in studies concerning cholesterol metabolism, immune responses, and inflammatory conditions. Researchers utilize this ELISA kit to quantify CH25H, thereby gaining valuable insights into its involvement in health and disease.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.