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Quantitative Rat VCAM1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1185

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative rat VCAM1 (glycosylated) sandwich ELISA kit is designed to detect rat vascular cell adhesion protein 1 (VCAM1) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 4.052 ng/mL, which can accurately detect low concentrations of VCAM1 in the sample.
Target
VCAM1
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated VCAM antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
4.052 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
16 ng/mL-1000 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Vascular cell adhesion protein 1
Alternate Names
Vascular cell adhesion protein 1; CD106 antigen; CD106; Vcam1; VCAM1
Uniprot No.
P29534
N-Glycosylation Site
273, 424, 531, 561, 650
Application Notes
Application
The quantitative rat VCAM1 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of VCAM1, helping to understand its role in signal transduction.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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