GlycoErase™ ACLY Knockout CHO-K1 Cell Line (CAT#: GLJF-0825-JF258)

Description	GlycoErase™ ACLY knockout CHO-K1 cell line is a Chinese hamster ovary (CHO-K1) model in which ACLY expression has been eliminated. ATP citrate lyase (ACLY) is the primary enzyme generating cytosolic acetyl-CoA from citrate, linking cellular energy metabolism to key biosynthetic pathways including lipogenesis and cholesterol synthesis. Loss of ACLY disrupts acetyl-CoA supply, thereby influencing lipid metabolism, membrane biosynthesis, and downstream regulatory processes. The knockout of ACLY in the CHO-K1 background provides a versatile system for studying acetyl-CoA-dependent pathways, metabolic flux, and lipid biosynthesis, while supporting applications in glycoengineering, biotherapeutic development, and metabolic research.
Product Type	KO Cell Lines
Species	Hamster
Cell Morphology	Epithelial, adherent
Passage Ratio	1:3~1:5
Cell Line	CHO-K1
Lineage	Chinese hamster ovary

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ACLY
Full Name	Atp Citrate Lyase
Alternative Name	ACL; ATPCL; CLATP
Location	17q21.2
Gene ID	47
Summary	ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.