GlycoErase™ ACLY Knockout CHO-K1 Cell Line

GlycoErase™ ACLY knockout CHO-K1 cell line is a Chinese hamster ovary (CHO-K1) model in which ACLY expression has been eliminated. ATP citrate lyase (ACLY) is the primary enzyme generating cytosolic acetyl-CoA from citrate, linking cellular energy metabolism to key biosynthetic pathways including lipogenesis and cholesterol synthesis. Loss of ACLY disrupts acetyl-CoA supply, thereby influencing lipid metabolism, membrane biosynthesis, and downstream regulatory processes. The knockout of ACLY in the CHO-K1 background provides a versatile system for studying acetyl-CoA-dependent pathways, metabolic flux, and lipid biosynthesis, while supporting applications in glycoengineering, biotherapeutic development, and metabolic research.

KO Cell Lines

Hamster

Epithelial, adherent

1:3~1:5

CHO-K1

Chinese hamster ovary

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.