GlycoErase™ ACLY Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF374)

Description	GlycoErase™ ACLY knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which ACLY expression has been eliminated. ATP citrate lyase (ACLY) is the primary enzyme generating cytosolic acetyl-CoA from citrate, linking cellular energy metabolism to biosynthetic pathways such as lipogenesis and cholesterol production. Loss of ACLY disrupts acetyl-CoA supply, reshaping lipid metabolism and membrane biogenesis in a human context. The knockout of ACLY in the HEK293 background provides a relevant system for studying metabolic rewiring in cancer cells, testing inhibitors of acetyl-CoA-dependent processes, and exploring links between lipid metabolism and gene regulation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ACLY
Full Name	Atp Citrate Lyase
Alternative Name	ACL; ATPCL; CLATP
Location	17q21.2
Gene ID	47
Summary	ATP citrate lyase is a tetrameric enzyme (~440 kDa) that generates cytosolic acetyl-CoA from citrate and CoA, with ATP hydrolysis to ADP and phosphate. Acetyl-CoA supports lipid and cholesterol synthesis, and may contribute to acetylcholine production in neural tissue. Multiple transcript variants encode distinct isoforms.