GlycoErase™ ACLY Knockout HEK293 Cell Line

GlycoErase™ ACLY knockout HEK293 cell line is a human embryonic kidney (HEK293) model in which ACLY expression has been eliminated. ATP citrate lyase (ACLY) is the primary enzyme generating cytosolic acetyl-CoA from citrate, linking cellular energy metabolism to biosynthetic pathways such as lipogenesis and cholesterol production. Loss of ACLY disrupts acetyl-CoA supply, reshaping lipid metabolism and membrane biogenesis in a human context. The knockout of ACLY in the HEK293 background provides a relevant system for studying metabolic rewiring in cancer cells, testing inhibitors of acetyl-CoA-dependent processes, and exploring links between lipid metabolism and gene regulation.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ACLY

Atp Citrate Lyase

ACL; ATPCL; CLATP

17q21.2

ATP citrate lyase is a tetrameric enzyme (~440 kDa) that generates cytosolic acetyl-CoA from citrate and CoA, with ATP hydrolysis to ADP and phosphate. Acetyl-CoA supports lipid and cholesterol synthesis, and may contribute to acetylcholine production in neural tissue. Multiple transcript variants encode distinct isoforms.