GlycoErase™ ADRB2 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF607)

Description	GlycoErase™ ADRB2 knockout HepG2 cell line eliminates ADRB2 expression in HepG2 cells. The beta-2-adrenergic receptor (ADRB2) is a GPCR that regulates calcium signaling, adenylyl cyclase activation, and cAMP-dependent kinase pathways. It also influences transcription of alpha-synuclein and is implicated in respiratory, cardiovascular, metabolic, and neurodegenerative disorders. This knockout supports studies of GPCR signaling, adrenergic pathway modulation, and receptor pharmacology in a hepatic context, with applications in drug discovery, metabolic research, and neurobiology.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	ADRB2
Full Name	Adrenoceptor Beta 2
Alternative Name	BAR; ARB2; B2AR; ADRBR; ADRB2R; BETA2AR
Location	5q32
Gene ID	154
Summary	This gene encodes the beta-2-adrenergic receptor, a G protein-coupled receptor directly linked to the class C L-type calcium channel Ca(V)1.2. The receptor forms a complex with a G protein, adenylyl cyclase, cAMP-dependent kinase, and phosphatase PP2A, enabling rapid and specific signal transmission. It also regulates alpha-synuclein transcription, with both genes linked to Parkinson's disease risk. This intronless gene shows variations and expression changes associated with nocturnal asthma, obesity, type 2 diabetes, and cardiovascular disease.