GlycoErase™ AGL Knockout Glycoengineered Cell

GlycoErase™ AGL knockout glycoengineered cell disrupts glycogen debrancher enzyme activity, which combines 4-alpha-glucotransferase and amylo-1,6-glucosidase functions critical for glycogen breakdown. Knocking out AGL allows exploration of glycogen metabolism, energy regulation, and disease mechanisms such as glycogen storage disorders. This model provides a tool for metabolic research and drug development.

KO Cell Lines

As requested by the client.

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

AGL

Amylo-Alpha-1, 6-Glucosidase, 4-Alpha-Glucanotransferase

GDE

1p21.2

This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described.