GlycoErase™ EPM2A Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF507)

Description	GlycoErase™ EPM2A knockout HEK293T cell line is a human embryonic kidney (HEK293T) model in which EPM2A (laforin) has been deleted. EPM2A encodes a dual-specificity phosphatase that prevents aberrant glycogen phosphorylation and aggregation. In HEK293T cells, knockout supports analysis of glycogen quality control, proteostasis-metabolism coupling, and pathways underlying Lafora disease, enabling target discovery and screening in a human system.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	EPM2A
Full Name	Epm2A Glucan Phosphatase, Laforin
Alternative Name	EPM2; MELF; MELF2
Location	6q24.3
Gene ID	7957
Summary	The product of this gene is a dual-specificity phosphatase that prevents over-phosphorylation of glycogen, thereby avoiding insoluble aggregate formation. Loss-of-function mutations lead to Lafora disease, a fatal epilepsy with Lafora body accumulation.