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GlycoErase™ HEK293F-ΔALG3 Cell Line

CAT#: GLJF-0425-JF259

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Overview Specification Target Information Related Products
Overview
Description
This ALG3 knockout (HEK293F) cell line lacks the ALG3 protein, an alpha-1,3-mannosyltransferase critical for N-linked glycosylation. It catalyzes the addition of the first mannose residue to the lipid-linked oligosaccharide precursor. Loss of ALG3 function can lead to congenital disorder of glycosylation type Id (CDG-Id), marked by abnormal N-glycosylation and potential developmental defects.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
HEK293F
Lineage
Embryonic kidney
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
ALG3
Full Name
ALG3 alpha-1,3- mannosyltransferase
Alternative Name
NOT56L, D16Ertd36e, CDGS4, Not56
Location
3q27.1
Gene ID
10195
Summary
Key player in N-glycosylation, adding alpha 1,3 mannose (dol-P-Man to Man5GlcNAc2-PP-Dol); disruptions linked to CDG-Id (congenital disorder of glycosylation type Id), causing abnormal N-glycosylation and potential neurological issues.
For Research Use Only.
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