GlycoErase™ ST3GAL1 Knockout CHO-K1 Cell Line

The ST3GAL1 knockout CHO-K1 cell line, obtained through gene knockout technology targeting ST3 beta-galactoside alpha-2,3-sialyltransferase 1, experimental results confirm that this cell line may cause severe damage to the critical α-2,3-sialylation modification pathway in CHO cells, as ST3GAL1 protein precisely catalyzes the transfer of sialic acid to galactose residues, playing a decisive role for large-scale preparation of high-quality therapeutic glycoprotein drugs' glycosylation precision control and process optimization.

KO Cell Lines

Hamster

Epithelial, adherent

1:3~1:5

CHO-K1

Chinese hamster ovary

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ST3GAL1

ST3 Beta-galactoside alpha-2,3-sialyltransferase 1

ST3GalA.1, ST3O, SIAT4A, SIATFL

8q24.22

Catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates; primarily located in the Golgi but can be processed into a soluble form.