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The STT3B knockout (CHO-K1) cell line, lacking STT3B oligosaccharyltransferase complex catalytic subunit B, may cause congenital disorders of glycosylation in CHO cells, given STT3B is vital for oligosaccharide transfer to asparagine and defects cause glycosylation disorders, offering a model to study severe glycosylation deficiencies in CHO bioproduction.
Product Type
KO Cell Lines
Species
Hamster
Cell Morphology
Epithelial, adherent
Passage Ratio
1:3~1:5
Cell Line
CHO-K1
Lineage
Chinese hamster ovary
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
FBS & Penicillin/Streptomycin & Proline & RPMI
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
STT3B
Full Name
STT3 oligosaccharyltransferase complex catalytic subunit B
Alternative Name
SIMP, STT3-B, FLJ90106
Summary
Catalytic subunit of a protein complex that transfers oligosaccharides to asparagine residues; defects cause congenital disorder of glycosylation Ix (CDG1X).