GlycoErase™ STT3B Knockout HepG2 Cell Line (CAT#: GLJF-0425-JF1125)

Description	STT3B knockout HepG2 cell line lacks STT3B protein, STT3 oligosaccharyltransferase complex catalytic subunit B, another essential OST subunit for N-glycosylation in liver cells. STT3B knockout in HepG2 cells enables study of N-linked glycosylation disruption in liver cancer, potentially causing congenital glycosylation disorders affecting hepatocytes and impacting N-glycan dependent processes in liver tumor development.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	STT3B
Full Name	STT3 oligosaccharyltransferase complex catalytic subunit B
Alternative Name	SIMP, STT3-B, FLJ90106
Location	3p23
Gene ID	201595
Summary	Catalytic subunit of a protein complex that transfers oligosaccharides to asparagine residues; defects cause congenital disorder of glycosylation Ix (CDG1X).