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GlycoFlux™ Human PDK3 Overexpression HepG2 Cell Line

CAT#: GLJF-0925-JF658

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Overview Specification Target Information Related Products
Overview
Description
GlycoFlux™ human PDK3 overexpression HepG2 cell line is a stable cell line engineered to express pyruvate dehydrogenase kinase 3 (PDK3), a heart- and muscle-enriched isoform that phosphorylates the E1α subunit of the PDH complex, thereby controlling the entry of glycolytic carbon into the TCA cycle. Several isoforms exist due to alternative splicing. This model enables studies of cardiac metabolism, muscle energetics, and the regulation of glucose utilization.
Product Type
Overexpression Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:4~1:6
Cell Line
HepG2
Primary Disease
Hepatoblastoma
Lineage
Liver
Lineage Subtype
Hepatoblastoma
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
qPCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Glutamine
Application
Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
PDK3
Full Name
Pyruvate Dehydrogenase Kinase 3
Alternative Name
CMTX6; GS1-358P8.4
Location
Xp22.11
Gene ID
5165
Summary
The protein encoded here is one of three pyruvate dehydrogenase kinases that inhibit the PDH complex by phosphorylating the E1 alpha subunit, thereby controlling the link between glycolysis and the TCA cycle. This gene is mainly expressed in heart and skeletal muscle. Several isoforms exist due to alternative splicing.
For Research Use Only.
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