GlycoFlux™ Human PPP1R3B Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF689)

Description	GlycoFlux™ human PPP1R3B overexpression HepG2 cell line is a stable cell line designed to express PPP1R3B, a PP1 regulatory protein expressed in liver and skeletal muscle. It influences glycogen synthesis and has been implicated in type 2 diabetes and MODY (maturity-onset diabetes of the young). Alternative splicing produces identical protein isoforms. This model provides a useful tool for metabolic disease studies, particularly glucose and glycogen regulation.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	PPP1R3B
Full Name	Protein Phosphatase 1 Regulatory Subunit 3B
Alternative Name	GL; PTG; PPP1R4
Location	8p23.1
Gene ID	79660
Summary	The encoded PP1 catalytic subunit is expressed in liver and skeletal muscle, with roles in glycogen regulation, and may be linked to type 2 diabetes and MODY. Alternative splicing yields identical proteins.