GlycoFlux™ Human PYGM Overexpression HEK293 Cell Line

GlycoFlux™ human PYGM overexpression HEK293 cell line is a stable cell line engineered to express PYGM, the muscle isoform of glycogen phosphorylase critical for glycogen breakdown in skeletal tissue. Variants in this gene underlie McArdle disease (glycogen storage disease V), a disorder of exercise intolerance and muscle energy failure. By modeling PYGM overexpression, this system provides a valuable tool to study muscle glycogen metabolism, energy regulation during contraction, and therapeutic approaches for metabolic myopathies.

Overexpression Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

qPCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & DMEM

Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

PYGM

Glycogen Phosphorylase, Muscle Associated

GSD5

11q13.1

This gene encodes the muscle isoform of glycogen phosphorylase, essential for glycogen breakdown in muscle tissue. Variants in this gene result in McArdle disease (myophosphorylase deficiency), a muscle glycogen storage disorder. Alternative splicing produces multiple transcript forms.